interleukin il 36γ  (Santa Cruz Biotechnology)

Journal: Journal of Inflammation Research

Article Title: Overexpression of Lin28a Aggravates Psoriasis-Like Phenotype by Regulating the Proliferation and Differentiation of Keratinocytes

doi: 10.2147/jir.s312963

Figure Lengend Snippet: Figure 2 Expression of differentiation markers and cytokines after IL-36γ treatment for the psoriasis-like condition in HaCaT. Mocks and Lin28a-overexpressing HaCaT were treated with recombinant human IL-36γ for 0, 6, and 12 h. Total RNAs were isolated from Mocks and Lin28a HaCaT cell lines treated with IL-36 γ and reverse transcribed; (A) relative mRNA expression levels of differentiation markers were measured by RT-PCR; and (B) relative mRNA expression levels of proinflammatory cytokines were examined through RT-PCR. Two-way ANOVA followed by Tukey’s multiple comparisons test was performed using GraphPad Prism 9. All data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared with each group.

Article Snippet: Then, 1 μg/mL recombinant human interleukin-36γ (IL36γ, R&D systems, MN, USA) was treated with Mock and Lin28a.

Techniques: Expressing, Recombinant, Isolation, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction